METHODS IN MOLECULAR BIOLOGY VOLUME 210 MHC PROTOCOLS

The aim of MHC Protocols is to document protocols that can be used for the analysis of genetic variation within the human major histocompatibility complex (MHC; HLA region). The human MHC encompasses approximately 4 million base pairs on the short arm of chromosome 6 at cytogenetic location 6p21. 3. The region is divided into three subregions. The telomeric class I region contains the genes that encode the HLA class I molecules HLA-A, -B, and -C. The centromeric class II region contains the genes encoding the HLA class II molecules HLA-DR, -DQ, and -DP. In between is the class III region, originally identified because it contains genes encoding components of the complement pathway. The entire human MHC has recently been sequenced (1) and each subregion is now known to contain many other genes, a number of which have immunological functions. The study of polymorphism within the MHC is well established, because the region contains the highly polymorphic HLA genes. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. As a result, large numbers of HLA alleles have been identified, a process that has been further driven by recent interest in HLA gene diversity in ethnic populations. The extreme genetic variation in HLA genes is believed to have been driven by the evolutionary response to infectious agents, but relatively few studies have analyzed associations between HLA genetic variation and infectious disease, which has been difficult to demonstrate. I. DatabasesHLA Informatics: Accessing HLA Sequences from Sequence DatabasesJames Robinson and Steven G. E. MarshAccessing HLA Sequencing Data Through the 6ace DatabaseRoger Horton and Stephan BeckII. Polymorphism in Classical and Nonclassical HLA GenesHLA Typing by Restriction Fragment Length Polymorphism AnalysisRobert W. VaughanPCR-Restriction Fragment Length Polymorphism Typing of Class I and II AllelesRobert W. VaughanPCR-Sequence-Specific Oliogonucleotide Probe Typing for HLA-A, -B, and -DRDerek Middleton and F. WilliamsHLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide ProbesLori L. Steiner, Priscilla V. Moonsamy, Teodorica L. Bugawan, and Ann B. BegovichPCR-Sequence-Specific Primer Typing of HLA Class I and Class II AllelesMike BunceHLA Typing With Reference Strand-Mediated Conformation AnalysisJ. Rafael Arguello, Martha Perez-Rodriguez, Andrea Pay, Gaby Fisher, Alasdair McWhinnie, and J. Alejandro MadrigalSequencing Protocols for Detection of HLA Class I PolymorphismPaul P. J. Dunn, Steven T. Cox, and Ann-Margaret LittleHLA-E and HLA-G TypingJorge Martinez-Laso, Eduardo Gomez-Casado, and Antonio Arnaiz-VillenaTyping Alleles of HLA-DMHelene TeisserencIII. Polymorphism in Non-HLA MHC GenesTyping Alleles of TAP1 and TAP2Stephen H. PowisDetermining Alleles of the C2 Gene by Southern BlottingZeng-Bian Zhu and John E. VolanakisComplement C4 Protein and DNA Typing MethodsPeter M. Schneider and Gottfried MauffTyping of Tumor Necrosis Factor AllelesAnthony Gerard WilsonMolecular Typing of the MHC Class I Chain-Related Gene LocusR. W. M. Collins, Henry A. F. Stephens, and Robert W. VaughanIV.MicrosatellitesHLA Microsatellite AnalysisMary CarringtonIndex